Ang II–stimulated migration of vascular smooth muscle cells is dependent on LR11 in mice
J. Clin. Invest. Meizi Jiang, et al. 118:2733 doi:10.1172/JCI32381 [
Go to this article.]
Figure 3Ang II–induced migration and attachment of cultured SMCs derived from
Lr11–/– mice.
(
A) Effect of Ang II on the PDGF-BB–induced (10 ng/ml) migration activities of
Lr11+/+ or
Lr11–/– SMCs. SMCs were incubated with 1 μM Ang II for 24 hours in the presence or absence of conditioned medium of
Lr11–/– SMCs before migration analyses. Data are presented as mean ± SD (
n = 6). (
B) Effect of Ang II on Stat1 phosphorylation in
Lr11+/+ or
Lr11–/– SMCs. SMCs were incubated with 1 μM Ang II for 10 minutes before immunoblot analysis for (phospho-) Stat1 (~90 kDa). (
C) Effects of Ang II on cell attachment of
Lr11+/+ or
Lr11–/– SMCs in the presence or absence of 10 ng/ml PDGF-BBs. SMCs were incubated with or without Ang II (1 μM) for 24 hours before attachment analyses. Data are presented as mean ± SD (
n = 6). (
D) Effects of sLR11 on Ang II–induced attachment of
Lr11+/+ or
Lr11–/– SMCs. SMCs were incubated with or without Ang II (1 μM) for 24 hours in the presence or absence of anti-LR11 antibody (pm11, 1:5 dilution) or recombinant sLR11 (1 μg/ml) for 24 hours before attachment analyses. Data are presented as mean ± SD (
n = 6). *
P < 0.05.
