A shed form of LDL receptor–related protein–1 regulates peripheral nerve injury and neuropathic pain in rodents
J. Clin. Invest. Alban Gaultier, et al. 118:161 doi:10.1172/JCI32371 [Go to this article.]

Figure 4
sLRP-α selectively regulates TNF-α–initiated cell signaling. Top: Protein extracts from Schwann cells in culture were subjected to SDS-PAGE and immunoblot analysis to detect phosphorylated ERK1/2 (P-ERK1/2) and total ERK1/2 (T-ERK1/2). The cells were first treated with sLRP-α (50 nM) or vehicle for 10 min prior to adding PDGF-BB (1.0 nM) for 10 min. Control cells were not treated with PDGF. Middle: Schwann cells were pretreated with sLRP-α (50 nM) or vehicle for 10 min and then with 1.0 nM erythropoietin (Epo) for 10 min. Immunoblot analysis was performed to detect P-JAK2 and β-actin. Bottom: Immunoblot analysis was performed to detect P-caveolin and β-actin. The cells were first treated with sLRP-α (50 nM) or vehicle for 10 min prior to adding 0.15 mM H₂0₂ for 10 min. The blots shown are representative of 2 independent studies.