A shed form of LDL receptor–related protein–1 regulates peripheral nerve injury and neuropathic pain in rodents
J. Clin. Invest. Alban Gaultier, et al. 118:161 doi:10.1172/JCI32371 [Go to this article.]

Figure 2
LRP1 α-chain is shed into Schwann cell CM. (A) Immunoblot analysis of cell extracts (cell) and CM after 6 h of incubation in serum-free medium. LRP1 α-chain (515 kDa) was detected using antibody 2629. LRP1 β-chain (85 kDa) was detected using antibody 11H4. The LRP1 α-chain also was detected by RAP ligand blotting. The mass of the band detected by RAP ligand blotting of cell extracts and CM was equivalent to that detected with antibody 2629. The same blots were probed for tubulin. These blots are representative of 3–5 independent studies. (B) SDS-PAGE and Coomassie staining of protein (sLRP-α) purified from human plasma by RAP-affinity chromatography. The same preparation was subjected to immunoblot analysis with antibody 2629.