Pomalidomide and lenalidomide regulate erythropoiesis and fetal hemoglobin production in human CD34⁺ cells
J. Clin. Invest. Laure A. Moutouh-de Parseval, et al. 118:248 doi:10.1172/JCI32322 [Go to this article.]

Figure 4
Pomalidomide modulates histone H3 acetylation on β-globin locus during erythroid differentiation of CD34⁺ cells. (A and B) CD34⁺ cells differentiated to the erythroid lineage in the presence of vehicle control or pomalidomide at 10 μM for 1 day, 3 days, or 6 days were subjected to ChIP using an anti-acetyl histone H3 K9 and K14 antibody and analyzed by RT-PCR using primers specific for Gγ globin, Aγ globin, and β promoters (A) and for LCR, HS2, and HS3 (B). Results are expressed as enrichment of genes of interest relative to the input DNA and are normalized to the results obtained with GAPDH-positive control. Values represent mean ± SEM (n = 3) and are representative of 2 independent experiments. (C) Acetylation of histone H3 on K9 and K14 was monitored by Western blot after 3 and 6 days of erythroid differentiation in the presence of vehicle control or pomalidomide at 10 μM. *P < 0.05; **P < 0.001.