Pomalidomide and lenalidomide regulate erythropoiesis and fetal hemoglobin production in human CD34⁺ cells
J. Clin. Invest. Laure A. Moutouh-de Parseval, et al. 118:248 doi:10.1172/JCI32322 [Go to this article.]

Figure 3
Pomalidomide modulates the transcription of β-globin locus genes. (A and B) Expanded CD34⁺ cells were differentiated to the erythroid lineage for 12 days in the presence of 1 μM pomalidomide. Cells were harvested at different times of erythroid differentiation, total RNA was extracted, and γ-globin (A) and β-globin (B) expression were monitored by real-time PCR analysis using Taqman PCR probes for γ-globin and β-globin, respectively. The quantity of γ-globin and β-globin RNA obtained was normalized to the quantity of β-actin RNA. (C and D) Gγ globin, Aγ globin, and β levels of transcription were assayed by ChIP. After 1, 3, or 6 days of cell differentiation in the presence of vehicle control or pomalidomide at 10 μM, chromatin was immunoprecipitated using anti-RNA pol II. Results are expressed as enrichment of Gγ globin, Aγ globin, and β genes relative to the input DNA and are normalized to the results obtained with GAPDH-positive control. Values represent mean ± SEM (n = 3) and are representative of 2 independent experiments. *P < 0.05; **P < 0.001.