Microvascular destruction identifies murine allografts that cannot be rescued from airway fibrosis
J. Clin. Invest. Ashok N. Babu, et al. 117:3774 doi:10.1172/JCI32311 [Go to this article.]

Figure 9
Neovascularization of membranous trachea by day 28 involves recipient-derived endothelium and supports a well-differentiated epithelium. (A) Neovascularization is evident by perfusion lectin staining in the membranous and intercartilaginous portions of the trachea following 28 days of rejection (n = 4). The dashed line indicates the anastomosis site separating the recipient and donor. (B) Use of FVB (Tie2/β-galactosidase) recipients (n = 3) demonstrated that by day 28, new vessels were composed partially of recipient-derived endothelium (large black arrows), and β-galactosidase expressing cells (small yellow arrow) appeared to still be present in the allograft in the proximity of vessels. (C) Five of 8 specimens studied demonstrate columnar epithelium only overlying the membranous portion of the trachea following 28 days of rejection, suggesting that the blood supply in this region may be required to support this epithelial phenotype. Original magnification, ×20 (B and C).