Microvascular destruction identifies murine allografts that cannot be rescued from airway fibrosis
J. Clin. Invest. Ashok N. Babu, et al. 117:3774 doi:10.1172/JCI32311 [
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Figure 8Additional transection of blood vessels during retransplantation affects perfusion and leads to fibrosis. As shown in Figure
7B, BALB/c→B6 allografts having undergone 6 days of rejection can be rescued from fibrosis by retransplantation into a naive B6 immunosuppressed hosts. (
A) The standard retransplantation technique involves cutting only the graft from the original recipient at the original anastomosis site and retransplanting it. (
B) Ten days following retransplant into naive immunosuppressed host, there is normal perfusion throughout the graft. (
C) By 28 days, histology is normal with no fibrosis and well-differentiated epithelium. (
D) To determine whether manipulating the blood flow to a rescuable airway could affect end outcome, we divided the day 6 allograft through the recipient ends, creating an additional transection of vessels. (
E) At 10 day following retransplant, there was no perfusion, in stark contrast to
B. (
F) By day 28, this airway had progressed to epithelial flattening and subepithelial fibrosis. Original magnification, ×20 (
C,
E, and
F).
