Microvascular destruction identifies murine allografts that cannot be rescued from airway fibrosis
J. Clin. Invest. Ashok N. Babu, et al. 117:3774 doi:10.1172/JCI32311 [Go to this article.]

Figure 4
Vascular perfusion is lost by day 10 in untreated allografts and is preserved in syngrafts and immunosuppressed allografts (n = 3 per box). (A) Normal vascular perfusion is maintained at day 8, day 10, and day 12 in syngrafts. However, there is complete loss of perfusion in allografts at day 10 and day 12. Perfusion of the vessels is completely preserved by immunosuppression at both day 10 and day 12. Treated allo, allograft treated with immunosuppression (anti–LFA-1 + anti-CD40L). (B) FITC–mean fluorescent intensity was measured as an index of blood flow, confirming the loss of blood flow at day 10 and day 12 in untreated allografts. MFI, mean fluorescent intensity (C) Using a mask based on FITC threshold to mark blood vessels, the percentage area of perfused vessels was assessed to confirm the histologic data. *P < 0.05 versus all other groups.