Elevation of RNA-binding protein CUGBP1 is an early event in an inducible heart-specific mouse model of myotonic dystrophy
J. Clin. Invest. Guey-Shin Wang, et al. 117:2802 doi:10.1172/JCI32308 [Go to this article.]

Figure 4
EpA960(R) mRNA forms foci that colocalize with MBNL1. In situ hybridization using a Cy3-CAG PNA probe was used to detect RNA foci. DAPI was used for nuclear staining. Heart tissue from the higher-expressing line EpA960/MCM 1323 (A) showed 8- to 10-fold more nuclei containing RNA foci than the lower-expressing line EpA960/MCM 1321 (B). (C) No signal was detected in cardiac tissue from the highest-expressing line using a Cy3-CTG (sense) probe or (D) in MCM mice treated with tamoxifen. (E) Endogenous MBNL1 colocalized with nuclear RNA foci in heart cells expressing EpA960(R) mRNA in line EpA960/MCM 1323. Original magnification, ×40 (A–D); ×63 (E).