Targeted gene deletion in Candida parapsilosis demonstrates the role of secreted lipase in virulence
J. Clin. Invest. Attila Gácser, et al. 117:3049 doi:10.1172/JCI32294 [
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Figure 6Phagocytosis and killing of
C. parapsilosis cells by macrophage-like cell lines J774.16 and RAW 264.7.
Fluorescent microscopic image of phagocytosis of
C. parapsilosis (
A) WT cells and (
B)
Δcplip1-2/ Δcplip1-2 lipase-negative cells by J774.16 macrophages. Dead
C. parapsilosis (arrowheads) show bright red and yellow fluorescence. Living cells show green fluorescence (arrows). Scale bars: 10 μm. (
C and
D) Phagocytosis by (
C) J774.16 and (
D) RAW 264.7 macrophage-like cells. Bars represent the mean measurements from 3 wells (at least 5 fields each). *
P < 0.02. (
E and
F) Intracellular killing of
C. parapsilosis WT and mutants by macrophage-like cells. Killing by (
E) J774.16 and (
F) RAW 264.7 macrophage-like cells. Bars represent mean percentage of killing determined by CFU counts. Error bars indicate SD. **
P < 0.01 (ANOVA).
