Targeted gene deletion in Candida parapsilosis demonstrates the role of secreted lipase in virulence
J. Clin. Invest. Attila Gácser, et al. 117:3049 doi:10.1172/JCI32294 [Go to this article.]

Figure 5
Reconstituted human oral epithelium at 48 hours infected with C. parapsilosis WT. (A and B) Uninfected control tissues, (C and D) WT, (E and F) heterozygous mutant CpLIP1-2/Δcplip1-2, (G and H) homozygous mutant Δcplip1-2/ Δcplip1-2, and (I and J) reconstituted Δcplip1-2/CpLIP2. Insets show histopathological alterations. Top insets in C and E show apoptotic cells; middle insets in C and E show intercellular edema; bottom inset in C shows cleft formation and tissue separation; and bottom inset in E shows vacuolization. Left panels demonstrate hematoxylin and eosin staining, and right panels periodic acid Schiff staining. Arrows indicate yeast cells, and arrowheads indicate pseudohyphae. Scale bars: 10 μm (A–J); 5 μm (all insets). (K) Relative LDH measured in the tissue culture supernatant by reconstituted human oral epithelium after 48 hours of coculture with WT, heterozygous mutant CpLIP1-2/Δcplip1-2, homozygous mutant Δcplip1-2/ Δcplip1-2, and reconstituted mutant Δcplip1-2/CpLIP2. Error bars indicate SD. The assay was repeated 3 times. *P < 0.02 (ANOVA).