Targeted gene deletion in Candida parapsilosis demonstrates the role of secreted lipase in virulence
J. Clin. Invest. Attila Gácser, et al. 117:3049 doi:10.1172/JCI32294 [Go to this article.]

Figure 4
Comparison of biofilm formed by C. parapsilosis WT and homozygous Δcplip1-2/Δcplip1-2, heterozygous CpLIP1-2/Δcplip1-2, and reconstituted Δcplip1-2/CpLIP2 mutants. (A) Light microscopy, (B) fluorescent microscopy, and (C) confocal microscopy images of WT biofilm on polyethylene surface after incubation for 48 hours at 37°C in YNB medium. The biofilm formed by the homozygous Δcplip1-2/Δcplip1-2 mutant is shown by (D) light microscopy, (E) fluorescent microscopy, and (F) confocal microscopy. (G–I) Comparison of biofilm formed by C. parapsilosis WT and lipase mutants on different surfaces. Relative XTT activity of biofilm formed on (G) polyethylene, (H) silicone, and (I) polystyrene surfaces. XTT activity was measured at 492 nm. *P < 0.02 (ANOVA). Error bars indicate SD. The experiment was repeated 3 times, and equivalent results were obtained. Scale bars: 50 μm (A, B, D, and E); 20 μm (C and F).