Monosaccharide-induced lipogenesis regulates the human hepatic sex hormone–binding globulin gene
J. Clin. Invest. David M. Selva, et al. 117:3979 doi:10.1172/JCI32249 [Go to this article.]

Figure 4
Insulin does not alter human SHBG production or HNF-4α protein levels in HepG2 cells cultured in the presence of high glucose. (A) Human SHBG accumulation in the medium is decreased in HepG2 cells treated daily with 1 mM or 10 mM glucose in the absence or presence of 6 μg insulin/ml for 5 days. Data points are shown as mean ± SD of triplicates; **P < 0.01 compared with no glucose supplementation. (B) Western blots of HNF-4α and cyclophilin A in HepG2 cells treated as in A for 5 days. (C) Western blots of phospho-S6 and cyclophilin A in HepG2 cells treated as in A.