Monosaccharide-induced lipogenesis regulates the human hepatic sex hormone–binding globulin gene
J. Clin. Invest. David M. Selva, et al. 117:3979 doi:10.1172/JCI32249 [
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Figure 3Monosaccharides decrease human SHBG production by HepG2 cells in concert with reduced cellular HNF-4α levels. (
A) Human SHBG accumulation in the medium of HepG2 cells treated daily with 10 mM of glucose or fructose was substantially reduced. Data points are shown as mean ± SD of triplicates; **
P < 0.01 compared with no glucose/fructose supplementation. (
B) Human SHBG and HNF-4α mRNA levels determined by RT-PCR in HepG2 cells cultured for 5 days as in
A. Cyclophilin A (CypA) mRNA was amplified as in internal control. (
C) HNF-4α and cyclophilin A levels measured by Western blotting in HepG2 cells cultured for 5 days as in
B. (
D) Human
SHBG promoter activity was measured in the context of a luciferase reporter gene assay in HepG2 cells cultured for 4 days in the presence or absence of 10 mM glucose or fructose. Data points are shown as mean ± SD of triplicates; **
P < 0.01 compared with no glucose/fructose supplementation.
