Monosaccharide-induced lipogenesis regulates the human hepatic sex hormone–binding globulin gene
J. Clin. Invest. David M. Selva, et al. 117:3979 doi:10.1172/JCI32249 [Go to this article.]

Figure 2
Insulin does not influence SHBG production by HepG2 cells. (A–D) HepG2 cells were cultured in serum-free medium, inactivated FBS, or untreated FBS for 5 days and treated daily with insulin at different concentrations. Accumulation of human SHBG in the medium was measured at timed intervals using an immunofluorometric assay (46) over a 5-day treatment period. Data points are shown as mean ± SD of triplicates; *P < 0.05, **P < 0.01 compared with no insulin treatment (A–C). On day 5 cells were taken for human SHBG mRNA and cyclophilin A mRNA measurements by RT-PCR analyses (D). (E) The effect of insulin (12 μg/ml) on human SHBG promoter activity was analyzed in HepG2 cells in the context of a luciferase reporter gene assay. Data points are mean ± SD of triplicate measurements.