Essential role of Skp2-mediated p27 degradation in growth and adaptive expansion of pancreatic β cells
J. Clin. Invest. Lingwen Zhong, et al. 117:2869 doi:10.1172/JCI32198 [
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Figure 5Deletion of
p27 reverses the morphological and metabolic phenotype of
Skp2–/– mice.
(
A and
B) Morphological analysis of pancreatic islets from 10-week-old
p27+/–Skp2–/– (
A) and
p27–/–Skp2–/– double-knockout (DKO;
B) mice. Pancreatic sections were stained with β-catenin (red) and DAPI (blue). White arrows point to the enlarged nuclear in
p27+/–Skp2–/– islets. Scale bars: 50 μm. (
C) Increase in β cell mass was greater in 10-week-old double-knockout mice than in
p27+/– Skp2–/– mice.
n = 3 per group. (
D) Impaired glucose tolerance was restored in 10-week-old double-knockout mice compared with that in
p27+/– Skp2–/– mice.
n = 3 per group. *
P < 0.05; ***
P < 0.005.
