Essential role of Skp2-mediated p27 degradation in growth and adaptive expansion of pancreatic β cells
J. Clin. Invest. Lingwen Zhong, et al. 117:2869 doi:10.1172/JCI32198 [
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Figure 3Decreased β cell mass, impaired glucose metabolism, and hypoinsulinemia in
Skp2–/– mice.
(
A) Analysis of β cell mass of
Skp2+/– and
Skp2–/– mice at 2 weeks, 10 weeks, and 6 months of age. Values are representative of 5 slides spanning the whole pancreas of each mouse and 3 mice per genotype at each age. (
B) Serum insulin levels in
Skp2+/– and
Skp2–/– mice before and 30 minutes after glucose injection i.p. following overnight fast.
n = 3 per group. (
C) Glucose tolerance test. Ten-week-old
Skp2+/– and
Skp2–/– mice were fasted overnight, and the blood glucose level was measured before and after glucose challenge. Differences were significant between groups at 30, 60, and 120 minutes (
P < 0.001 for all comparisons).
n = 5 (
Skp2+/–); 8 (
Skp2–/–). *
P < 0.05; ***
P < 0.005.
