Essential role of Skp2-mediated p27 degradation in growth and adaptive expansion of pancreatic β cells
J. Clin. Invest. Lingwen Zhong, et al. 117:2869 doi:10.1172/JCI32198 [
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Figure 1Deletion of
Skp2 resulted in the accumulation of p27 protein in pancreatic islet cells.
(
A and
B) Pancreatic sections from 3-week-old
Skp2+/– (
A) and
Skp2–/– (
B) mice were immunostained with anti-mouse insulin (green) and anti-mouse p27 (red) antibodies. Original magnification, ×20. Arrows in
A show β cells that do not express p27 in wild-type islets. Arrows in
B show high levels of accumulated p27 in β cells in
Skp2–/– islets. (
C) Immunoblot analysis of p27 and phospho-p27 (p-p27) expression in the islet cells isolated from
Skp2+/– and
Skp2–/– pancreata. Graph demonstrates densitometric analysis of immunoblot of p27 and phospho-p27 normalized to tubulin. For all panels, data are representative of 3 experiments.
