A Foxo/Notch pathway controls myogenic differentiation and fiber type specification
J. Clin. Invest. Tadahiro Kitamura, et al. 117:2477 doi:10.1172/JCI32054 [
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Figure 2Quantitative analysis of C2C12 differentiation. (
A) Western blotting analysis of myosin expression in C2C12 cells. (
B) Morphometric analysis of myosin-positive cells. Results from differentiation experiments were analyzed by scoring the number of myosin-immunostained cells as a percentage of all DAPI-positive cells. (
C) DBD-Foxo1-ADA reporter gene assays. We carried out reporter gene assays using the canonical Foxo1-responsive
Igfbp1 promoter (left panel) and the
Hes1 promoter (right panel) in cells cotransfected with Foxo1-ADA or DBD-Foxo1-ADA. Western blot (inset) demonstrates that expression levels of the 2 proteins are similar. An asterisk indicates
P < 0.01 by ANOVA.
