Proteolytic processing of dynamin by cytoplasmic cathepsin L is a mechanism for proteinuric kidney disease
J. Clin. Invest. Sanja Sever, et al. 117:2095 doi:10.1172/JCI32022 [
Go to this article.]
Figure 1CatL is essential for proteinuric kidney diseases. (
A) Quantitative rt-PCR of microdissected glomeruli from human biopsies of patients with acquired proteinuric diseases: minimal change disease (MCD;
n = 7), membranous glomerulonephritis (MGN;
n = 9), focal segmental glomerulosclerosis (FSGS;
n = 7), and diabetic nephropathy (DN;
n = 10). **
P < 0.01. CON, control (
n = 8). (
B) CatL labeling of normal human kidney. (
C) CatL labeling of human kidney with diabetic nephropathy, mildly reduced renal function, and nephrotic range proteinuria. (
D) Immunocytochemistry of mouse glomeruli using monoclonal anti-CatL antibody. WT mice received either PBS (WT CON) or LPS (WT LPS). LPS was also injected into CatL
–/– mice (CatL
–/– LPS). Original magnification, ×400 (
C and
D). (
E) Electron micrographs of FPs. (
F) Urinary protein levels determined using the standard Bradford protein assay. Urine was collected immediately before (Baseline) and 48 hours after addition of LPS. Each bar represents at least 8 animals.
