Parasympathetic response in chick myocytes and mouse heart is controlled by SREBP
J. Clin. Invest. Ho-Jin Park, et al. 118:259 doi:10.1172/JCI32011 [Go to this article.]

Figure 6
I_KACh is markedly decreased in atrial myocytes from SREBP-1 KO mice compared with WT and increased by overexpression of SREBP-1c. (A) I_KACh is decreased in dissociated atrial myocytes from SREBP-1 KO mice compared with cells from atria of WT mice, as described in Methods. I_KACh was determined as described in Figure 1A. (B) I-V plots constructed from a series of data points in A, panel iii. Data represent the mean of 7 determinations from 3 separate preparations. (C) I-V plots from dissociated myocytes from atria of SREBP-1 KO mice infected with an adenovirus expressing GFP or GFP plus SREBP-1c (MOI of 20). Forty-eight hours after infection, I_KACh was determined in GFP⁺ cells by fluorescence microscopy (90% of cells) as in A and B. Points are the mean of 7 determinations from 3 separate cultures. (D) Mean peak inward current from 7 determinations; *P < 0.001 compared with WT; ^†P < 0.004 compared with KO.