Parasympathetic response in chick myocytes and mouse heart is controlled by SREBP
J. Clin. Invest. Ho-Jin Park, et al. 118:259 doi:10.1172/JCI32011 [Go to this article.]

Figure 2
Cis-acting elements required for SREBP regulation of the GIRK1 promoter. (A) Specificity of SREBP stimulation of GIRK1 promoter activity. Atrial myocytes were cotransfected with a construct containing 1.6 kb of the GIRK1 promoter reporter and increasing amounts of a construct expressing either SREBP-1a, -1c, or -2. (B) Upper panel: Nucleotide sequence of the GIRK1 promoter from –1070 to –1209. Lower panel: Deletion constructs of the GIRK1 promoter. (C and D) Effect of deletion mutants of the GIRK1 promoter on SREBP-1a stimulation of promoter activity. Data are plotted as fold stimulation above basal level of the p30 promoter in the absence of SREBP-1a, taken as 1. Data represent the mean of 4 determinations carried out in triplicate. Note that scales of the y axes in C and D are different. (E) Effect of FX deletion in the GIRK1 promoter on LPDS stimulation of promoter activity. Cells cultured in FBS or LPDS were transfected with either the full-length GIRK1 promoter (p30) or the FX deletion mutant. The activity of the p30 promoter in FBS was considered as 1. *P < 0.05, **P < 0.001 compared with control.