Pten controls lung morphogenesis, bronchioalveolar stem cells, and onset of lung adenocarcinomas in mice
J. Clin. Invest. Shigehisa Yanagi, et al. 117:2929 doi:10.1172/JCI31854 [Go to this article.]

Figure 5
Spontaneous lung adenocarcinomas and urethane-induced lung tumors in SOPten^flox/flox mice that received doxycycline postnatally. (A) Gross and histological analyses of spontaneous adenocarcinomas. Left and middle: gross appearance of a healthy lung in a 48-week-old WT(P21–P27) mouse and representative lung adenocarcinomas (arrow) in a lung of a 48-week-old SOPten^flox/flox(P21–P27) mouse. Right: histology of a spontaneous lung adenocarcinoma in the lung of a 48-week-old SOPten^flox/flox(P21–P27) mouse, representative of adenocarcinomas observed in 13/15 of these mice. Scale bar: 50 μm. (B) Gross and histological analyses of urethane-induced tumors. WT(P21–P27) (n = 18) and SOPten^flox/flox(P21–P27) mice (n = 15) were injected i.p. with urethane. After 20 weeks, the number of tumors and their diameters were measured from the lung surface. Left and middle: gross appearance of urethane-induced lung tumors (arrows) in WT(P21–P27) and SOPten^flox/flox(P21–P27) mice. Right: histology of a lung adenoma representative of those observed in both WT(P21–P27) and SOPten^flox/flox(P21–P27) mice. Scale bar: 50 μm. (C) Numbers and mean size of urethane-induced tumors in WT(P21–P27) and SOPten^flox/flox(P21–P27) mice. Horizontal bars in the left panel represent the mean tumor numbers for the WT(P21–P27) and SOPten^flox/flox(P21–P27) groups. The right panel shows the mean diameter ± SD of tumors in WT(P21–P27) and SOPten^flox/flox(P21–P27) lungs. *P < 0.001; **P < 0.005, Welch’s t test.