Pten controls lung morphogenesis, bronchioalveolar stem cells, and onset of lung adenocarcinomas in mice
J. Clin. Invest. Shigehisa Yanagi, et al. 117:2929 doi:10.1172/JCI31854 [Go to this article.]

Figure 3
Altered gene expression profile during lung morphogenesis and distal epithelial cell differentiation in SOPten^flox/flox(E10–E16) lungs. (A) RT-PCR analyses of the mRNA expression of the indicated genes in lungs of WT(E10–E16) and SOPten^flox/flox(E10–E16) mice at E19.5. Spry2 and Shh are differentially expressed. GAPDH was used as a loading control. Data shown are representative of 3 trials. (B) IHC analysis of Spry2 and Shh expression in lungs of WT(E10–E16) and SOPten^flox/flox(E10–E16) mice at E19.5. A dramatic upregulation of Spry2 and Shh in the alveolar epithelium in SOPten^flox/flox(E10–E16) lungs was observed. Scale bars: 50 μm.