Enhanced at puberty 1 (EAP1) is a new transcriptional regulator of the female neuroendocrine reproductive axis
J. Clin. Invest. Sabine Heger, et al. 117:2145 doi:10.1172/JCI31752 [Go to this article.]

Figure 2
The EAP1 gene is expressed in subregions of the nonhuman primate and rat hypothalamus involved in reproductive control. (A) Low-magnification image showing that EAP1 mRNA expression is prominent in cells of the ARC of the primate hypothalamus as assessed by in situ hybridization using a monkey-specific [³⁵S]UTP-labeled EAP1 cRNA probe. The section shown is from an 8-year-old adult female monkey. ME, median eminence of the hypothalamus; OC, optic chiasm; V, third ventricle. Scale bar: 300 μm. (B) Montage image of the monkey hypothalamus (adult, 8-year-old female monkey) showing that EAP1 immunoreactive protein is preferentially localized to cells of the ARC. The dotted line outlines the medial and lateral ARC. Scale bar: 200 μm. (C) High-magnification view of A showing that EAP1 mRNA is localized to cell nuclei that appear to be neuronal because they are lightly stained with thionin (examples denoted by arrows). Scale bar: 20 μm. (D) High-magnification view of the ARC showing immunoreactive EAP1 in neuronal nuclei. Scale bar: 20 μm. (E) Low-magnification view of the MBH from a 28-day-old prepubertal female rat showing EAP1 staining in cell nuclei of the ARC and ventromedial (VMH) nuclei (outlined by dotted lines). The immunohistochemical reaction was developed to a brown color with diaminobenzidine hydrochloride (Supplemental Note 3), and the cell nuclei were counterstained with 0.1% methyl green. (F) Lack of EAP1 staining in an adjacent section incubated with EAP1 antibodies preadsorbed with the antigenic peptide used to prepare the antibodies (10 μg peptide/ml antiserum, overnight at 4°C). (G) Higher-magnification view of the ARC showing EAP1 staining (brown) in cell nuclei. Some cells did not contain EAP1, so their nuclei have only a green color. (H) Adjacent section stained with peptide-preadsorbed EAP1 antibodies. Scale bars: 100 μm (in E and F); 20 μm (G and H).