CX3CR1-dependent subretinal microglia cell accumulation is associated with cardinal features of age-related macular degeneration
J. Clin. Invest. Christophe Combadière, et al. 117:2920 doi:10.1172/JCI31692 [Go to this article.]

Figure 2
The M280 polymorphism leads to CX3CR1 dysfunction and impaired migration. (A) Representative CCL2-dependent migration through CX3CL1-coated filters of monocytes from individuals homozygous for the 2 extreme haplotypes of CX3CR1 (VT and IM). Flow cytometry was used to count the number of CD14-positive cells that migrated into the lower chamber. Each data point is the mean ± SEM of 3 different determinations. (B) Genetic variations of CX3CR1-impaired CCL2-dependent migration through filters coated with CX3CL1. Monocytes from individuals homozygous for CX3CR1-IM variants (n = 5) have less CCL2-dependent chemotactic ability in the presence of a CX3CL1-coated filter than do monocytes from individuals homozygous for CX3CR1-VT variants (n = 8). Migration ability is expressed as the ratio of the chemotactic index (CI) in response to CCL2 of monocytes migrating through a filter with or without a CX3CL1 coating (chemotactic index without CX3CL1 relative to chemotactic index with CX3CL1). ***P = 0.0006.