An erythroid chaperone that facilitates folding of α-globin subunits for hemoglobin synthesis
J. Clin. Invest. Xiang Yu, et al. 117:1856 doi:10.1172/JCI31664 [Go to this article.]

Figure 3
Blocked erythroid maturation in double-mutant mice. (A) The developmental stage of splenic erythroid precursors was analyzed by flow cytometry according to a protocol described by Liu et al (31). Ter119⁺ erythroid progenitors were fractionated by forward scattering and CD71 expression into increasingly mature populations, termed Ery.A, Ery.B, and Ery.C. Results of representative experiments on wild-type and double-mutant mice are shown. (B) Two to 5 mice of each genotype were analyzed at age 6 months. The y axis shows frequencies of spleen erythroblast subsets as percentage of total Ter119⁺ cells for each genotype indicated on the x axis. Each symbol represents data for a single mouse. The horizontal bar indicates the mean value. The differences between single and double mutants were statistically significant (2-tailed P value): Ery.B: Ahsp^–/– versus Ahsp^–/–α-globin*^α/αα, P < 0.005; α-globin*^α/αα versus Ahsp^–/–α-globin*^α/αα, P < 0.001; Ery.C: Ahsp^–/– versus Ahsp^–/–α-globin*^α/αα, P < 0.08; α-globin*^α/αα versus Ahsp^–/–α-globin*^α/αα, P < 0.003.