Ewing sarcoma gene EWS is essential for meiosis and B lymphocyte development
J. Clin. Invest. Hongjie Li, et al. 117:1314 doi:10.1172/JCI31222 [
Go to this article.]
Figure 2EWS is intrinsically required for pre–B cell development. (
A) A complete blood count was performed with peripheral blood from 3-week-old littermates (
n = 3/genotype). White blood cell differential data indicate a marked reduction of the lymphocyte population in
Ews–/– mice. (
B) Reduced cellularity of lymphoid organs in
Ews–/– mice. Total cell count is shown for indicated organs of 3-week-old mice (
n = 3/genotype). Flow cytometry of splenocytes (
C) and bone marrow–derived cells (
D) from
Ews–/– mice and littermate controls using B cell markers B220, CD43, IgM, and IgD. (
E) Fetal liver chimera analysis. Bone marrow cells were harvested from fetal liver chimeras and analyzed by flow cytometry with antibodies against CD43 and B220 along with CD45.1 and CD45.2. Presented are representative data from 3 independent experiments with similar results (
n = 3 for
Ews–/– and
n = 3 for
Ews+/– littermate controls). (
F) CFU assay. Bone marrow cells from fetal liver chimeras were plated in duplicate in either pre-B or multilineage differentiation (GM-CFU) media. The number of colonies is presented as the mean colony number from 3 independent experiments.
