Multipotent adult progenitor cells sustain function of ischemic limbs in mice
J. Clin. Invest. Xabier L. Aranguren, et al. 118:505 doi:10.1172/JCI31153 [
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Figure 2mMAPC-VP and mBMC engraftment and differentiation. (
A–
C) At 14 days, mMAPC-VP were lower in number compared with mMAPC-U (compare
A and Figure
1C). Confocal imaging revealing more GFP
+ cells coexpressing CD31 (
B) and α-SMA (arrowheads in
C). Overexposure in the DAPI channel was used to reveal muscle tissue in
A. (
D–
G) At 14 days, anti-GFP staining revealed that mBMCs engrafted mostly in connective and fat tissue outside muscle (
D) and only occasionally intercalated between muscle cells (
E). Confocal analysis showing that mBMCs (green;
F) did not integrate in but were located outside (arrowheads in
F) vessels (CD31; red;
F) and that most of the cells (green;
G) colocalized (yellow;
G) with the pan-hematopoietic marker CD45 (red;
G). Images in
A–
D,
F, and
G are from adductor; the image in
E is from gastrocnemius muscle. Topro was used as nuclear counterstain in
F. Scale bars: 40 μm (
B and
C), 62.5 μm (
F and
G), 100 μm (
D and
E), and 150 μm (
A).
