MFG-E8–mediated uptake of apoptotic cells by APCs links the pro- and antiinflammatory activities of GM-CSF
J. Clin. Invest. Masahisa Jinushi, et al. 117:1902 doi:10.1172/JCI30966 [Go to this article.]

Figure 5
GM-CSF regulates CD4⁺ effector T cells through MFG-E8. (A) Peritoneal macrophages were exposed to apoptotic thymocytes and cocultured with syngeneic wild-type CD3⁺CD45RA⁺CD62^hi naive T cells. T cells were then analyzed for IL-17 and IFN-γ production. Numbers refer to the percentage of cells within an indicated gate. (B) Flt3-L–derived bone marrow dendritic cells were exposed to apoptotic thymocytes and used to stimulate syngeneic wild-type naive T cells. (C) Engineered macrophages were exposed to apoptotic thymocytes and used to stimulate syngeneic wild-type naive T cells. (D). Splenocytes were stimulated with PMA and ionomycin, and cytokine production of gated CD3⁺CD4⁺ cells was determined by flow cytometry. Similar results were obtained in at least 2 experiments.