Inhibition of TGF-β with neutralizing antibodies prevents radiation-induced acceleration of metastatic cancer progression
J. Clin. Invest. Swati Biswas, et al. 117:1305 doi:10.1172/JCI30740 [
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Figure 5TGF-β–neutralizing antibody 2G7 blocks radiation-induced increase in lung metastases. (
A and
B) Eight-week-old tumor-bearing
MMTV/PyVmT mice were administered 10 Gy to the thorax. Where indicated, mice were treated with 15 mg/kg of 2G7 twice a week until week 13, at which time surface lung metastases were counted (
A). Data are mean ± SD of 5 mice per group. (
B) Representative H&E stains of lung sections. The experiment was repeated once with similar results. (
C) Blood was collected at the completion of the experiment via heart puncture and its cellular fraction evaluated for its ability to form colonies ex vivo as described in Methods. (
D) At 13 weeks, blood was collected from tumor-bearing transgenic mice that were exposed to thorax irradiation. The cellular fraction was plated ex vivo as in
C in the presence of 20 μg/ml 2G7 or PBS. Colonies measuring 50 μm or greater were counted manually 10–12 days later. Data are mean ± SD of 5 mice per group. *
P < 0.05, **
P < 0.01 versus control.
