AFAP-110 is overexpressed in prostate cancer and contributes to tumorigenic growth by regulating focal contacts
J. Clin. Invest. Jing Zhang, et al. 117:2962 doi:10.1172/JCI30710 [Go to this article.]

Figure 3
Effects of AFAP-110 downregulation on the ability to form colonies on soft agar. The colony-forming assay was performed by seeding equal amount of cells of different groups in soft agar in 6-well tissue culture plates in triplicate. Numbers of colonies with a diameter greater than 20 μm were quantified after 2 weeks. (A) Representative images of colonies in soft agar. Scale bars: 50 μm. (B) Numbers of colonies per well are expressed as means and 95% CIs of experiments performed in triplicate (2 independent experiments were performed); bars correspond to 95% CI. *P < 0.001, 2-tailed Student’s t test.