Homeostatically proliferating CD4⁺ T cells are involved in the pathogenesis of an Omenn syndrome murine model
J. Clin. Invest. Khie Khiong, et al. 117:1270 doi:10.1172/JCI30513 [Go to this article.]

Figure 6
MM mice have symptoms similar to those of OS patients. (A) The percentage of eosinophils (CCR3^highGr-1^low), as determined using PBLs of wild-type and MM mice, was greater in MM than in wild-type mice. Results are mean and SD of 12 mice. (B) To examine the skin of wild-type and MM mice, their abdominal hair was shaved. Panel shows the skin color of wild-type and MM mice, typical of more than 10 analyzed. (C–F) Weights of the thymus (C), spleen (D), lymph nodes (E), and liver (F) at the indicated time points were higher in MM than in wild-type mice. Results are mean and SD of more than 50 mice. (G) Vβ usage by CD4⁺ and CD8⁺ T cells in 8-week-old wild-type and MM mice was measured by FACS analysis. Results are mean and SD of 5 mice. The percentage of Vβ3⁺, Vβ4⁺, Vβ5.1⁺5.2⁺, Vβ7⁺, Vβ10⁺, Vβ12⁺, and Vβ13⁺ CD4⁺ cells and of Vβ5.1⁺5.2⁺, Vβ7⁺, Vβ10⁺, Vβ12⁺, Vβ13⁺, and Vβ14⁺ CD8⁺ cells was lower, while the percentage of Vβ6⁺, Vβ8.1⁺8.2⁺, Vβ8.3⁺, Vβ9⁺, and Vβ11⁺ CD4⁺ cells and of Vβ6⁺, Vβ8.1⁺8.2⁺, and Vβ11⁺ CD8⁺ cells was higher, in MM compared with wild-type mice. (H) Spectratypes of Vα and Vβ family genes were performed using splenocytes from MM mice and C57BL/10 controls. Representative data are shown. *P < 0.05, ^#P < 0.01, ^†P < 0.001 versus wild type.