Hypoxia promotes fibrogenesis in vivo via HIF-1 stimulation of epithelial-to-mesenchymal transition
J. Clin. Invest. Debra F. Higgins, et al. 117:3810 doi:10.1172/JCI30487 [
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Figure 1Hypoxia induces morphological and phenotypic changes in PTECs consistent with an EMT phenotype. PTECs cultured under hypoxic (1% O
2; right panels) compared with normoxic (21% O
2; left panels) conditions displayed altered cell shape (phase contrast, original magnification, ×100), reduced staining for the epithelial junction protein ZO-1 (green; original magnification, ×400), and increased staining for α-SMA (red; original magnification, ×200), nuclei were stained with DAPI (blue).
