Role of Gas6 in erythropoiesis and anemia in mice
J. Clin. Invest. Anne Angelillo-Scherrer, et al. 118:583 doi:10.1172/JCI30375 [
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Figure 6Impaired Epo survival response of
Gas6–/– erythroblasts.
(
A and
B) TUNEL staining of spleen 3 days after induction of hemolytic anemia by PHZ. Black staining with TUNEL denotes localized apoptotic cells in WT (
A) and Gas6
–/– (
B) spleen. F, follicle; RP, red pulp. Scale bars: 100 μm. (
C) TUNEL assay of WT and
Gas6–/– erythroblasts isolated 6 days after PHZ injection. Cells were cultured for 16 h in the presence or absence of recombinant human Epo at the indicated concentrations. The TUNEL assay was quantified by flow cytometry (
n = 4); results are expressed as the percentage of apoptotic erythroblasts, cells double-stained for TUNEL and Ter-119. (
D) Dead cell count of splenic erythroblasts (Trypan blue
+ cells) collected on day 6 after PHZ injection and maintained in culture for 24 h in the presence or absence of Epo (1.5 IU/ml) or Epo plus human rGas6 (400 ng/ml).
n = 8 per group. Values are mean ± SEM. *
P < 0.05 versus WT.
