Role of Gas6 in erythropoiesis and anemia in mice
J. Clin. Invest. Anne Angelillo-Scherrer, et al. 118:583 doi:10.1172/JCI30375 [
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Figure 4Expression of Gas6 and Gas6Rs in erythroblasts. (
A) Western blot analysis of Axl and Mertk in spleen erythroblasts isolated from WT,
Tyro3–/–,
Axl–/–, and
Mertkkd mice. Equal amounts of protein cell lysates were loaded per well. (
B) Erythropoiesis of
Tyro3–/–,
Axl–/– or
Mertkkd mice in response to PHZ. Overall genotypic differences versus WT (after Dunnett’s correction for multiple testing) were as follows:
Tyro3–/–,
P = 0.49 (
n = 18);
Axl–/–,
P = 0.007 (
n = 9);
Mertkkd,
P = 0.97 (
n = 5). (
C) WT and
Axl–/– mice were subjected to PHZ-induced hemolytic anemia (injections of a half dose of PHZ on days 0 and 1) and treated with saline or rGas6 (2 μg daily intraperitoneally).
P < 0.05,
Axl–/– and
Axl–/– plus rGas6 versus WT;
P > 0.05,
Axl–/– versus
Axl–/– plus rGas6.
