Dependence of intestinal granuloma formation on unique myeloid DC-like cells
J. Clin. Invest. Atsushi Mizoguchi, et al. 117:605 doi:10.1172/JCI30150 [Go to this article.]

Figure 3
Granuloma-associated CD11c⁺ cells exhibit a unique IMD-like phenotype with F4/80 expression. (A) Staining of CD11c versus CD11b (left panel) and B220 or CD8α expression (solid red histograms, right panels) on gated CD11b⁺CD11c⁺ cells from grossly recognizable granulomas (n = 12) are shown. (B) Expression (n = 9) of F4/80 or MOMA-1 on gated CD11b⁺CD11c⁺ cells are shown as solid green histograms. Black lines represent isotype control staining. (C) QPCR shows F4/80 expression by purified CD11c⁺ cells (n = 3) from granulomas and splenic CD4⁺ cells (n = 3). (D) Morphology of FACS-purified F4/80⁺CD11c⁺ cells from granulomas is shown. Fine dendrites are recognized on these cells (arrows). (E) Expressions of CD86 on granuloma-derived CD11c⁺CD11b⁺ cells (solid orange histograms) and BM-derived mature DCs (blue line) are shown. Black line represents isotype control staining. The averages of CD86 mean fluorescent intensity (MFI) on BM-derived immature (iDC, n = 3) and mature (mDC, n = 3) and granuloma-derived CD11c⁺CD11b⁺ cells (gDC, n = 10) are summarized. (F) T cells (T) from BALB/c mice were cultured without or with C57BL/6 mDC or gDC (n = 3) for 80 hours and pulsed with 1 μCi ³H for 16 hours. (G) iDC (n = 3), mDC (n = 3), and gDC (n = 3) were cultured with FITC-dextran at 4°C or 37°C and subjected to flow cytometric analysis. The averages of phagocytic activity are indicated as FITC-dextran MFI at 37°C minus FITC-dextran MFI at 4°C. Bottom panel shows gDC cultured with FITC-dextran at 37°C. Statistical significances are indicated by asterisks. **P < 0.001; *P < 0.05.