An essential role for Notch in neural crest during cardiovascular development and smooth muscle differentiation
J. Clin. Invest. Frances A. High, et al. 117:353 doi:10.1172/JCI30070 [Go to this article.]

Figure 3
DNMAML-GFP is activated specifically in neural crest and somites by Pax3-Cre and does not affect neural crest cell number or migration. (A and B) E10.5 Pax3^Cre/+ Z/EG control (A) and Pax3^Cre/+ DNMAML mutant (B) embryos demonstrating expression of DNMAML-GFP in pharyngeal arches (arrows) and somites (arrowheads). (C and D) Immunostaining for GFP with Hoechst nuclear counterstain on frontal sections through the pharyngeal arches of E10.5 Pax3^Cre/+ DNMAML embryos. (C) Low-magnification view showing GFP-positive cells investing the third, fourth, and sixth aortic arch arteries. (D) Higher magnification showing GFP expression specifically in the neural crest–derived mesenchyme of the pharyngeal arch (nc), but not in pharyngeal epithelium (ep) or endothelial cells (ec). (E and F) Immunostaining for GFP on frontal sections through the conotruncus of E11.5 embryos, showing an equivalent number of GFP-positive cells in the conotruncal cushions (arrows) of control (E) and mutant (F) embryos. (G–J) Immunostaining for GFP and α-SMA on frontal sections through the aortic arch arteries of E11.5 embryos. (G and H) Low-magnification view showing equivalent numbers of GFP-positive cells in the pharyngeal region surrounding the 6 major aortic arch arteries (arrows) in control (G) and mutant (H) embryos. (I and J) High-magnification views of the left sixth aortic arch arteries shown in G and H. (K–N) In situ hybridizations for the neural crest cell marker PlexinA2 on frontal sections through the conotruncus (K and L) and the aortic arch arteries (M and N) of E11.5 embryos, showing equivalent expression in control (K and M) and mutant (L and N) embryos. (O and P) GFP expression in the mature aortic arch of control Pax3^Cre/+R26R^GFP (O) and mutant Pax3^Cre/+ DNMAML (P) mice. Scale bars: 100 μm (C, E–H, and K–N), 20 μm (D).