Hepatic Niemann-Pick C1–like 1 regulates biliary cholesterol concentration and is a target of ezetimibe
J. Clin. Invest. Ryan E. Temel, et al. 117:1968 doi:10.1172/JCI30060 [Go to this article.]

Figure 3
Hepatic expression of NPC1L1 results in a dramatic reduction in biliary cholesterol but not PL and BA. (A) Concentration of gallbladder cholesterol, PL, and BA in male mice was determined as described in Methods. Molar ratios of each lipid in total biliary lipids were calculated from lipid concentrations. Data are mean ± SEM of 4–6 samples. (B and C) Liver total mRNAs or membrane proteins were prepared as described previously (50) from mice in A, and equal amounts of mRNA or protein from each mouse within a group were pooled (n = 5 per group). Levels of selected mRNAs and proteins were determined by quantitative real-time PCR (B) and by immunoblotting (C), respectively, as described previously (10, 50). RAP was used as the loading control, and human NPC1L1 was immunoblotted with L1Ab for genotype validation. The experiment was repeated twice with different sets of animals, and similar results were obtained. P < 0.05 between * and ^# groups for each measurement (ANOVA).