Real-time optical recording of β₁-adrenergic receptor activation reveals supersensitivity of the Arg389 variant to carvedilol
J. Clin. Invest. Francesca Rochais, et al. 117:229 doi:10.1172/JCI30012 [Go to this article.]

Figure 4
Supersensitivity of the Arg389–β₁-AR polymorphism to carvedilol. (A) Representative tracings of the beating frequency of cardiomyocytes infected with the Gly389–β₁-AR sensor or the Arg389–β₁-AR sensor before and after stimulation with carvedilol (1 μM). The number of beats per minute was determined from the digitized video images by an observer who was blinded to the experimental protocol. (B) Effect of carvedilol (1 μM) on the beating frequency of cardiomyocytes infected with LacZ, the Gly389–β₁-AR sensor, or the Arg389–β₁-AR sensor (5 independent cardiomyocyte isolations each, including 5–10 measurements for each of the 6 groups). **P < 0.01 and ***P < 0.001, statistically significant differences. Ctrl, control.