Disruption of LDL but not VLDL clearance in autosomal recessive hypercholesterolemia
J. Clin. Invest. Christopher Jones, et al. 117:165 doi:10.1172/JCI29415 [Go to this article.]

Figure 3
Clearance of circulating VLDL but not LDL is partially preserved in Arh^–/– mice. (A) Clearance of mouse ¹²⁵I-VLDL and rabbit ¹²⁵I–β-VLDL in Arh^–/–, Ldlr^–/–, and wild-type mice. Four wild-type (squares), Arh^–/– (diamonds), and Ldlr^–/– (circles) 12- to 14-week-old female mice were injected with mouse ¹²⁵I-VLDL (125 cpm/ng protein) (left) or rabbit ¹²⁵I–β-VLDL (288 cpm/ng protein) (right). Blood samples were collected by retro-orbital puncture at the indicated times, and the plasma content of isopropanol-precipitable ¹²⁵I-radioactivity was measured. Radioactivity remaining in the plasma was plotted as a percentage of the activity present 2 minutes after injection of the labeled ligand. Before the experiment, the mice were fasted for 6 hours and anesthetized with sodium pentobarbital (80 mg/kg intraperitoneal). (B) Female Arh^+/+Ldlr^h/+ (squares), Arh^–/–Ldlr^h/+ (diamonds), and Ldlr^–/– (circles) mice (n = 4 per genotype), aged 12–14 weeks, were fasted for 4 hours, anesthetized with sodium pentobarbital (80 mg/kg of intraperitoneal), and injected with 15 μg ¹²⁵I-human LDL (125 cpm/ng protein) (left) or 15 μg ¹²⁵I-rabbit β-VLDL (189 cpm/ng protein) (right). Blood samples were collected and processed as described above.