Embryonic endocrine pancreas and mature β cells acquire α and PP cell phenotypes upon Arx misexpression
J. Clin. Invest. Patrick Collombat, et al. 117:961 doi:10.1172/JCI29115 [
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Figure 3Quantification of the phenotypic alterations in hormone-producing cell contents following the targeted misexpression of
Arx.
Six-week-old independent pancreata estimated to be of the same size were serially sectioned, every tenth section was stained as indicated, and the numbers of positive cells were counted and reported to the total islet cell content (estimated on adjacent sections with the use of a mixture of antibodies raised against the different endocrine hormones). Data are shown as the percentage ± SEM of hormone-positive cells contributing to the total endocrine population. On average, the misexpression of
Arx during early pancreas genesis promotes a dramatic loss of β and δ cells, whereas the contents in glucagon- and PP-labeled cells are proportionally increased. *
P < 0.05, **
P < 0.01, ***
P < 0.001.
n ≥ 8.
