Hyperactivation of p21^ras and PI3K cooperate to alter murine and human neurofibromatosis type 1–haploinsufficient osteoclast functions
J. Clin. Invest. Feng-Chun Yang, et al. 116:2880 doi:10.1172/JCI29092 [Go to this article.]

Figure 2
Nf1^+/– osteoclasts have elevated Ras and92 PI3K activity. (A) Ras activity in WT and Nf1^+/– osteoclasts was measured at the indicated times following stimulation with M-SCF. A representative blot is shown (left panel). The relative increase in Ras activity in Nf1^+/– osteoclasts compared with WT osteoclasts in 4 independent experiments is indicated (right panel). Data represent mean ± SEM. *P < 0.01, Ras activity of Nf1^+/– osteoclasts versus WT osteoclasts. (B) Akt phosphorylation was measured at 0 and 2 minutes following stimulation with M-CSF. Results from 1 of 4 independent experiments are shown (left panel), and the relative increase (mean ± SEM; n = 4) in Akt phosphorylation in Nf1^+/– osteoclasts compared with WT osteoclasts is indicated (right panel). **P < 0.01, Akt phosphorylation of Nf1^+/– osteoclasts versus WT osteoclasts. (C) Erk phosphorylation was measured at the indicated levels following stimulation with M-CSF. A representative Western blot (left panel) is shown, and the relative mean increase (mean ± SEM; n = 4) in Erk phosphorylation in Nf1^+/– osteoclasts is indicated (right panel). ^†P < 0.01, Nf1^+/– osteoclasts versus WT osteoclasts.