Hyperactivation of p21^ras and PI3K cooperate to alter murine and human neurofibromatosis type 1–haploinsufficient osteoclast functions
J. Clin. Invest. Feng-Chun Yang, et al. 116:2880 doi:10.1172/JCI29092 [Go to this article.]

Figure 1
Nf1^+/– mice have increased numbers of osteoclast progenitors and osteoclasts in vivo. Data in B, C, and E represent the mean ± SEM of 5 independent experiments. (A) Clonogenic assays were established to determine the number of CFU-Ms per femur. Representative TRAP^– CFU-M (left panel) and TRAP⁺ CFU-M (right panel). (B) Total CFU-Ms per femur from Nf1^+/– or WT mice. *P < 0.01, Nf1^+/– CFU-Ms versus WT CFU-Ms by Student’s t test. (C) TRAP⁺ CFU-Ms per femur from mice of the indicated genotypes. *P < 0.01, Nf1^+/– versus WT. (D) Representative photomicrographs (magnification, ×10) of WT and Nf1^+/– distal femoral metaphyses following TRAP staining. Arrows indicate selected osteoclasts. (E) Average size of osteoclasts from Nf1^+/– and WT mice. Ten high-power fields per experimental mouse were scored. **P < 0.05, Nf1^+/– versus WT osteoclasts.