Indoleamine 2,3-dioxygenase–expressing dendritic cells form suppurative granulomas following Listeria monocytogenes infection
J. Clin. Invest. Alexey Popov, et al. 116:3160 doi:10.1172/JCI28996 [
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Figure 2Overall transcriptional changes in human DCs infected with
L. monocytogenes.
(
A) Unsupervised hierarchical clustering analysis of immDCs infected with
L. monocytogenes (L.m.) for 2 hours or 6 hours or control (Con) mock-infected DCs, cultured at the same conditions for 2 hours or 6 hours. Affymetrix raw data were normalized using dChip, and a list of 1189 genes with high variability across the data set (0.5 < SD/mean < 10) was used for hierarchical clustering in dChip. (
B) Visualization using GeneSpring software of the most significantly changed genes after infection with
L. monocytogenes. A total of 902 transcripts were defined with dChip as significantly changed at either time point; filtering criteria are described in detail in Methods. These significantly changed genes were ordered according to the temporal pattern of expression changes.
Immediate-response genes are defined as being either up- or downregulated at the 2-hour time point but not at the 6-hour time point.
Immediate-early response genes are characterized by upregulated or downregulated genes at both time points, and
early response genes are regulated only at 6 hours. Expression values were normalized and color coded with upregulated genes in red and downregulated genes in blue. Each colored curve represents an expression pattern of a single gene; each vertical line represents 1 sample as indicated at the bottom of the figure. Detailed gene information is provided in Supplemental Table 2.
