Indoleamine 2,3-dioxygenase–expressing dendritic cells form suppurative granulomas following Listeria monocytogenes infection
J. Clin. Invest. Alexey Popov, et al. 116:3160 doi:10.1172/JCI28996 [Go to this article.]

Figure 1
Human DCs in L. monocytogenes infection. (A) Histomorphology of lymph node sections from a patient with suppurative granulomatous listeriosis. H&E staining of a sample slide with prominent ring-wall formation of histiocytoid cells around suppurative granulomas in listeriosis. Magnification, ×100 and ×400 (H&E panels). Photographs of immunohistochemical staining with CD20, CD3, CD15, CD68, S100, CD11c, and IDO are shown. Magnification, ×250. One out of 3 comparable cases is depicted here. (B) Immunofluorescence of the same patient samples described above. S100 and CD68 were combined to distinguish between DCs and macrophages. S100 and CD11c were combined to determine a myeloid origin of the S100⁺ DCs. As a second marker for macrophages, double staining with CD68 and CD11c was performed. To assess expression of IDO by myeloid cells, DCs, and macrophages, analysis of IDO was combined with S100, CD11c, or CD68. One out of 3 cases is presented. Magnification, ×250. In each lower-left corner, an enlarged section of the photo is shown for more detail (magnification, ×2500). (C) Confocal microscopy (Olympus FluoView FV1000 Confocal Microscope) clearly confirms the colocalization of IDO with S100 and CD68. Magnification, ×2500.