PDGFRs are critical for PI3K/Akt activation and negatively regulated by mTOR
J. Clin. Invest. Hongbing Zhang, et al. 117:730 doi:10.1172/JCI28984 [Go to this article.]

Figure 3
mTOR activation inhibits signaling of both PDGF and EGF to Akt. MEFs were serum starved for 24 hours and then stimulated with 25 ng/ml PDGFbb or 50 ng/ml EGF for 10 minutes. Cell lysates were then subjected to Western blot analysis. (A) Note similar patterns of p-ERK increase in response to PDGF and EGF in both WT and Tsc2^–/– cells. In contrast, p-Akt levels were reduced in response to both EGF and PDGF in the Tsc2^–/– cell line. (B) Note enhancement of p-Akt levels in Tsc2^–/– cells in response to rapamycin (100 nM for 24 hours) and PDGF or EGF treatment.