PDGFRs are critical for PI3K/Akt activation and negatively regulated by mTOR
J. Clin. Invest. Hongbing Zhang, et al. 117:730 doi:10.1172/JCI28984 [Go to this article.]

Figure 2
mTOR suppresses PDGFR expression. (A and B) Immunoblot analysis is shown for Pten^–/–, Tsc1^–/– (A), and Tsc2^–/– (B) MEFs. (A) Cells were treated with 10 nM rapamycin for variable time periods, up to 24 hours. (B) Cells were starved in DMEM with rapamycin for 24 hours; treated with or without 0.1 μM wortmannin for 30 minutes; then stimulated with 10% FCS (left) or 50 ng/ml PDGFbb (right) for 10 minutes. Note that rapamycin immediately decreased p-S6 levels and led to a delayed increase in IRS1 and PDGFRβ levels. (C) Real-time quantitative RT-PCR analysis of PDGFRα and PDGFRβ mRNA levels is shown for untreated and 24-hour rapamycin–treated (10 nM) Tsc1^–/– and WT MEFs. Error bars indicate SD for n = 2 experiments. Note the marked decrease in PDGFR mRNA levels in Tsc1^–/– cells, which were increased by rapamycin treatment.