Human CD4⁺ CD25^hi Foxp3⁺ regulatory T cells are derived by rapid turnover of memory populations in vivo
J. Clin. Invest. Milica Vukmanovic-Stejic, et al. 116:2423 doi:10.1172/JCI28941 [Go to this article.]

Figure 4
Regulatory CD4⁺ CD25^hi T cells are closely related to effector CD4⁺ T cells. CD4⁺CD25^hi and CD4⁺CD25^– cells were FACS sorted as before, and heteroduplex analysis was performed for 26 Vβ families. (A) Heteroduplex analysis of CD4⁺CD25^– and CD4⁺CD25^hi cells showing a representative Vβ family from 1 out of the 7 volunteers that were investigated. Solid arrows on the left of the blot indicate clonal bands that are present in both subsets. The asterisk indicates the position of the carrier homoduplexes. The schematic representation of the distribution of clones present in CD4⁺CD25^– and CD4⁺CD25^hi subpopulations across the 26 Vβ families in 1 individual is shown in B, where the presence of a clone in a particular Vβ family for either subset is represented by a symbol and a clone that is shared between the 2 subsets is represented by a line joining 2 symbols. (C) Schematic representation of the clones present in CD4⁺CD25^hi population from 1 donor at different time points taken 7 months apart.