A p53-derived apoptotic peptide derepresses p73 to cause tumor regression in vivo
J. Clin. Invest. Helen S. Bell, et al. 117:1008 doi:10.1172/JCI28920 [Go to this article.]

Figure 3
37AA binds to iASPP and disrupts its interaction with p73. (A) 37AA binds iASPP. Saos-2 cells were infected with adenoviruses expressing GFP, wtp53, tr105, tr210, and 37AA following transfection with Flag-iASPP. Cells were lysed and split into 2 and used for either Western analysis (GFP blot) or IP (Flag-iASPP pulldown and GFP blot). WB, Western blot. (B) 37AA does not affect iASPP protein levels. Cells were transfected with iASPP and either GFP, 37AA, or empty vector. Protein levels were measured by Western blotting. (C and D) 37AA affects iASPP localization and dissociates iASPP from p73. Cells were transfected with iASPP, GFP, 37AA, or p73. (C) iASPP and GFP localization was determined by immunofluorescence. (D) iASPP binding to p73 was determined by IP.